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Product Specifications
Analytical sensitivity
0.08 ng/mL
Assay range
1.56-75 ng/mL
Sample type/volume
Plasma
25 µL
Serum
25 µL
Hands-on time
1 hr 20 min
Time-to-result
2 hr 30 min
Homogenous (no wash)
No
Interassay CV
4.6%
Intraassay CV
3.9%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Standard Dilution Buffer
Incubation Buffer
HRP conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Controls
Standard
Standard Dilution Buffer
Incubation Buffer
HRP conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Controls
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
Osteocalcin
Protein family
Hormones & Receptors
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
HRP
Label or dye
HRP
About This Kit
The Human Osteocalcin ELISA quantitates Hu Osteocalcin in human serum and plasma,. The assay will exclusively recognize both natural and recombinant Hu Osteocalcin.
Principle of the method
The Human Osteocalcin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
Osteocalcin belongs to the osteocalcin/matrix Gla-protein family and constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Gene aliases : BGLAP, BGP, OC, OCN
Gene ID : (Human) 632
Gene symbol : BGLAP
Protein Aliases : BGP, bone gamma-carboxyglutamate (gla) protein (osteocalcin), Bone Gla protein, Gamma-carboxyglutamic acid-containing protein, Osteocalcin
UniProt ID (Human) P02818
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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