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Product Specifications
Analytical sensitivity
0.2 ng/mL
Assay range
1.2-75 ng/mL
Sample type/volume
Plasma
25 µL
Serum
25 µL
Supernatant
25 µL
Hands-on time
15 min
Time-to-result
2 hr 10 min
Homogenous (no wash)
No
Interassay CV
8.1%
Intraassay CV
8.3%
Instrument
Colorimetric Microplate Reader
Product size
128 Tests
Contents
Pre-coated 96 well Microplate
Detection Antibody
Anti-IgG-HRP
Sample Diluent, lyophilized
Standard (8-well strips x 4)
Wash Buffer Concentrate
Substrate Solution
Sample Diluent
Stop Solution
Lysis Buffer 10x
Adhesive Plate Covers
Detection Antibody
Anti-IgG-HRP
Sample Diluent, lyophilized
Standard (8-well strips x 4)
Wash Buffer Concentrate
Substrate Solution
Sample Diluent
Stop Solution
Lysis Buffer 10x
Adhesive Plate Covers
Shipping conditions
Dry ice
Storage
-20°C
Protein name
Osteocalcin
Species (tested)
Human
Assay kit format
Instant ELISA Kit
Detector antibody conjugate
HRP
Label or dye
HRP
About This Kit
The Human Osteocalcin ELISA quantitates Hu Osteocalcin in human serum, plasma, cell culture supernatants, urine, amniotic fluid, bile, or other body fluids. The assay will exclusively recognize both natural and recombinant Hu Osteocalcin.
Principle of the method
The Human Osteocalcin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
Osteocalcin belongs to the osteocalcin/matrix Gla-protein family and constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : BGLAP, BGP, OC, OCN
Gene ID : (Human) 632
Gene symbol : BGLAP
Protein Aliases : BGP, bone gamma-carboxyglutamate (gla) protein (osteocalcin), Bone Gla protein, Gamma-carboxyglutamic acid-containing protein, Osteocalcin
UniProt ID (Human) P02818
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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