Technical guide & protocol Tech Support

Product Specifications

Analytical sensitivity

0.20 ng/mL

Assay range

0.63-40.00 ng/mL

Sample type/volume

Amniotic Fluid
10 µL
Plasma
10 µL
Serum
10 µL
Supernatant
10 µL

Hands-on time

1 hr 20 min

Time-to-result

2 hr 30 min

Homogenous (no wash)

No

Interassay CV

5.4%

Intraassay CV

7.8%

Instrument

Colorimetric Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate
Standard
Sample Diluent
Assay Buffer concentrate
HRP-conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Controls
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

2-8°C

Protein name

P-Selectin (CD62P)

Target sub-specificity

Soluble

Species (tested)

Human

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

HRP

Label or dye

HRP

About This Kit

The Human Soluble P-Selectin (Hu sP-Sel/CD62)) ELISA quantitates Hu sP-Selectin in human serum, plasma, amniotic fluid, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu sP-Selectin.

Principle of the method

The Human sP-Selectin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Target information

P-selectin (Selectin P, GMP-140, LECAM3, CD62 antigen-like family member) is a 140 kDa type 1 transmembrane glycoprotein, and is one of the most commonly studied proteins that regulate cell rolling. P-selectin is stored in the Weibel-Palade bodies of endothelial cells, as well as in a-granules of platelets. From there, it can be rapidly brought to the cell surface after exposure to thrombin, histamine, complement 5a, Ca21 ionophores, or adenosine diphosphate. P-selectin protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. P-selectin plays an important role in adhesive processes between leucocytes and endothelial cells, and is a calcium dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens found on neutrophils and monocytes. P-selectin is constitutively expressed in inflammation and contributes to atherogenesis, thrombosis and tissue destruction. Clinically, P-selectin is used to distinguish heparin induced thrombocytopenia with and without thrombosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Gene aliases : CD62, CD62P, GMP140, GMRP, GRMP, LECAM3, PADGEM, PSEL, SELP

Gene ID : (Human) 6403

Gene symbol : SELP

Protein Aliases : CD62 antigen-like family member P, CD62P, GMP-140, Granule membrane protein 140, granule membrane protein 140kDa, granulocyte membrane protein, LECAM3, Leukocyte-endothelial cell adhesion molecule 3, P-selectin, PADGEM, Platelet activation dependent granule-external membrane protein, platelet alpha-granule membrane protein, selectin P (granule membrane protein 140kDa, antigen CD62)

UniProt ID (Human) P16109

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