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Product Specifications
Analytical sensitivity
2 pg/mL
Assay range
2.74-2000 pg/mL
Sample type/volume
Plasma
1 µL
Serum
1 µL
Supernatant
100 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 45 min
Homogenous (no wash)
No
Interassay CV
<12%
Intraassay CV
<10%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
PARC
Protein family
Chemokines & Receptors
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Human PARC/CCL18 ELISA quantitates Hu PARC in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu PARC.
Principle of the method
The Human PARC solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
Macrophage Inflammatory Protein-4 (MIP-4), also called CCL18, is a chemokine expressed in the lymph nodes, lungs, placenta and bone marrow. Although the MIP-4s receptor remains undetermined, MIP-4 is known to act as a chemoattractant for activated and non-activated T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : AMAC-1, AMAC1, CCL18, CKb7, DC-CK1, DCCK1, MIP-4, MIP4, PARC, SCYA18
Gene ID : (Human) 6362
Gene symbol : CCL18
Protein Aliases : Alternative macrophage activation-associated CC chemokine 1, AMAC-1, C-C motif chemokine 18, CC chemokine ligand 18, CC chemokine PARC, chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated), chemokine (C-C), dendritic, DC-CK1, Dendritic cell chemokine 1, Macrophage inflammatory protein 4, MIP-4, Pulmonary and activation-regulated chemokine, small inducible cytokine A18, small inducible cytokine subfamily A (Cys-Cys), member 18, pulmonary and activation-regulated, Small-inducible cytokine A18
UniProt ID (Human) P55774
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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