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Product Specifications
Analytical sensitivity
<0.6 pg/mL
Assay range
4.69-300 pg/mL
Sample type/volume
Serum
50 µL
Plasma
50 µL
Cell Lysate
50 µL
Supernatant
50 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr
Homogenous (no wash)
No
Interassay CV
3.4%
Intraassay CV
2.1%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Biotinylated Detection antibody
Sample Diluent
Assay Buffer concentrate
Wash Buffer concentrate
SAV-HRP
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Biotinylated Detection antibody
Sample Diluent
Assay Buffer concentrate
Wash Buffer concentrate
SAV-HRP
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
PD-L1 (CD274)
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
HRP
Label or dye
HRP
About This Kit
The Human Programmed T cell Death Ligand 1 (Hu PD-L1) ELISA quantitates Hu PD-L1 in human serum, plasma, cell lysates or cell culture medium. The assay will exclusively recognize both natural and recombinant H PD-L1.
Principle of the method
The Human PD-L1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Target information
Programmed death receptor ligand 1 (PD-L1, also called B7-H1) is a recently described B7 family member. To date, one specific receptor has been identified that can be ligated by PD-L1. This receptor, programmed death receptor 1 (PD-1), has been shown to negatively regulate T-cell receptor (TCR) signaling. Upon ligating its receptor, PD-L1 has been reported to decrease TCR-mediated proliferation and cytokine production. PD-L1 expression was found to be abundant on many murine and human cancers and could be further up-regulated upon IFN-gamma stimulation. Thus, PD-L1 might play an important role in tumor immune evasion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : B7-H, B7H1, CD274, PD-L1, PDCD1L1, PDCD1LG1, PDL1
Gene ID : (Human) 29126
Gene symbol : CD274
Protein Aliases : B7 homolog 1, B7-H1, CD274, CD274 antigen, PD-L1, PDCD1 ligand 1, Programmed cell death 1 ligand 1
UniProt ID (Human) Q9NZQ7
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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