Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
The Human Free Prostate Specific Antigen/Kallikrein 3 (Hu freePSA/KLK3) ELISA quantitates Hu free PSA/KLK3 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu free PSA/KLK3.
Principle of the method
The Human free PSA/KLK3 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
PSA is a chymotrypsin-like serine protease (kallikrein family) produced by the prostate epithelium, and is abundant in seminal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liver-derived serine protease inhibitor, alpha-1-antichymotrypsin (ACT). A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign prostate hyperplasia. PSA is used to confirm prostatic acinar cell origin in primary and metastatic carcinoma and to rule out non-prostatic carcinoma mimics.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Gene aliases : APS, hK3, KLK2A1, KLK3, PSA
Gene ID : (Human) 354
Gene symbol : KLK3
Protein Aliases : Gamma-seminoprotein, Kallikrein-3, kallikrein-related peptidase 3, P-30 antigen, prostate specific antigen, Prostate-specific antigen, PSA, Seminin, Semenogelase
UniProt ID (Human) P07288
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