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Product Specifications
Analytical sensitivity
11.8 pg/mL
Assay range
28.1-1800 pg/mL
Sample type/volume
Serum
20 µL
Plasma
20 µL
Cell Lysate
20 µL
Hands-on time
1 hr 20 min
Time-to-result
3 hr 30 min
Homogenous (no wash)
No
Interassay CV
9.3%
Intraassay CV
4.9%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard (2 vials)
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
Streptavidin-HRP
Wash Buffer
Substrate Solution
Stop Solution
Adhesive Plate Covers
Standard (2 vials)
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
Streptavidin-HRP
Wash Buffer
Substrate Solution
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
Perforin
Target sub-specificity
Soluble
Protein subtype
Adaptor Proteins
Protein family
Cytolytic Protein
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Perforin Human ELISA Kit quantitates Hu Perforin in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Perforin.
Principle of the method
The Human Perforin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
Perforin is one of the major cytolytic proteins of cytolytic granules. Perforin is a cytolytic mediator and is stored in and released by cytoplasmic granules. Moreover, perforin is involved in immune defense against tumors and virus infections as mediated by cytotoxic lymphocytes. Perforin is a 555 amino acid protein with a 21 amino acid signal peptide, and has a molecular weight of 70 to 75 kD. Perforin is a pore forming protein with a mechanism of transmembrane channel formation similar to C9, and homology between perforin and C9 have been demonstrated. Studies show that perforin is expressed only in killer cell lines and not in helper T lymphocytes or other tumor cells tested. Perforin is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in the perforin gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants of perforin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Gene aliases : FLH2, HPLH2, P1, PFN1, PFP, PRF1
Gene ID : (Human) 5551
Gene symbol : PRF1
Protein Aliases : Cytolysin, cytolysin, lymphocyte pore-forming protein, perforin 1 (pore forming protein), P1, PFP, HPLH2, lymphocyte pore forming protein, Lymphocyte pore-forming protein, Perforin-1
UniProt ID (Human) P14222
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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