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Product Specifications
Analytical sensitivity
Lot-specific
Assay range
Lot-specific
Hands-on time
1 hr 45 min
Time-to-result
24 hr 30 min
Homogenous (no wash)
No
Instrument
Colorimetric Microplate Reader
Product size
10 x 96 Tests
Contents
This Human RANKL (RANK Ligand) ELISA Kit includes Capture Antibody, Detection Antibody, Protein Standard, and HRP-conjugate. The HRP-conjugate is packaged separately.
Storage
Refer to product documentation for component specific storage temperature.
Protein name
RANKL (RANK Ligand)
Protein family
Growth Factors & Receptors
Species (tested)
Human
Assay kit format
ELISA Development Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
900-K142K consists of: 900-K142 (capture antibody, detection antibody, and protein standard), and AVIDIN-60UL (avidin HRP-conjugate).
Human RANKL (RANK Ligand) ABTS ELISA Development Kit contains the key components required for the quantitative measurement of natural and/or recombinant human RANKL (RANK Ligand) in a sandwich ELISA format. Human RANKL (RANK Ligand) ABTS ELISA Development Kit contains enough material to assay the target cytokine in at least 1,000 ELISA plate wells.
The ABTS ELISA Buffer Kit (Product # 900-K00), sold separately, is recommended to be used in conjunction with this ABTS ELISA Development Kit for optimal performance. This buffer kit contains all the necessary components to assay ten 96-well ELISA plates (included) and contains detailed handling instructions.
Target information
This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : CD254, hRANKL2, ODF, OPGL, OPTB2, RANKL, sOdf, TNFSF11, TNLG6B, TRANCE
Gene ID : (Human) 8600
Gene symbol : TNFSF11
Protein Aliases : CD254, ODF, OPGL, Osteoclast differentiation factor, Osteoprotegerin ligand, RANKL, receptor activator of nuclear factor kappa B ligand, Receptor activator of nuclear factor kappa-B ligand, TNF-related activation-induced cytokine, TRANCE, tumor necrosis factor (ligand) superfamily member 11, tumor necrosis factor (ligand) superfamily, member 11, tumor necrosis factor ligand 6B, Tumor necrosis factor ligand superfamily member 11
UniProt ID (Human) O14788
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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