Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
The Human SAA ELISA Kit quantifies human serum amyloid A in serum, plasma, buffered solution, or cell culture medium. The assay will recognize both the natural 18 kDa isoform and recombinant SAA.
Principle of the method
The Human SAA solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
The serum amyloid A family comprises a number of differentially expressed apolipoproteins, acute-phase SAA1 and SAA2, the former being the major component in plasma and constitutive SAAs. Although the liver is the primary site of synthesis of both SAA types extrahepatic production has been reported. The in vivo concentrations increase by as much as 1000 fold during inflammation. Several studies have expressed its importance in the diagnosis and monitoring of various diseases. Pathological SAA values are often detected in association with normal CRP concentrations; SAA rises earlier and more sharply than CRP. Recently, a broader view of SAA expression and function has been emerging.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Gene aliases : PIG4, SAA, SAA1, SAA2, TP53I4
Gene ID : (Human) 6288
Gene symbol : SAA1
Protein Aliases : SAA, Amyloid fibril protein AA, serum amyloid A protein, Serum amyloid A-1 protein, tumor protein p53 inducible protein 4
UniProt ID (Human) P0DJI8
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