Technical guide & protocol Tech Support

Product Specifications

Analytical sensitivity

144.21 U/mL

Assay range

234-15,000 Units/mL

Sample type/volume

Serum
5 µL
Plasma
5 µL

Hands-on time

30 min

Time-to-result

1 hr 15 min

Homogenous (no wash)

No

Interassay CV

<10%

Intraassay CV

<10%

Instrument

Colorimetric Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate
High Control (standard), Calibrator (medium control), Low Control
IgG - HRP conjugate
Assay Buffer concentrate
Wash Buffer concentrate
Substrate solution
Stop Solution
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

2-8°C

Protein name

SARS-CoV-2 Spike (trimer) Protein

Species (tested)

Human

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

The Human SARS-CoV-2 Spike (Trimer) IgG ELISA is a serology assay that measures and quantitates immunoglobulin G (IgG) antibodies against SARS-CoV-2 Spike (Trimer) in human serum or plasma.

Principle of the method

The Human SARS-CoV-2 Spike (Trimer) IgG ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of IgG antibodies bound to SARS-CoV-2 Spike (Trimer). A trimerized Spike protein is pre-coated in the wells of the supplied microplate. Samples, controls, including a high control that can be used as a standard are then added into these wells and bind to the immobilized (capture) Spike protein. The wells are washed and IgG antibodies conjugated to HRP are added and will bind to any captured antibodies. The wells are washed and a substrate solution is added that reacts with the enzyme complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of antibody present in the original specimen.

Target information

It has recently been shown that SARS (severe acute respiratory syndrome) is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness, such as the common cold, in humans. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The glycosylated spike protein (as well as the nucleocapsid protein) can be detected in infected cell culture supernatants with antisera from SARS patients.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?
Let us know so we can reference it here.
Cite this product
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support