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Product Specifications
Analytical sensitivity
Lot-specific
Assay range
Lot-specific
Hands-on time
1 hr 45 min
Time-to-result
24 hr 30 min
Homogenous (no wash)
No
Instrument
Colorimetric Microplate Reader
Product size
10 x 96 Tests
Contents
This Human SCF ELISA Kit includes Capture Antibody, Detection Antibody, Protein Standard, and HRP-conjugate. The HRP-conjugate is packaged separately.
Storage
Refer to product documentation for component specific storage temperature.
Protein name
SCF
Protein family
Growth Factors & Receptors
Species (tested)
Human
Assay kit format
ELISA Development Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
900-K34K consists of: 900-K34 (capture antibody, detection antibody, and protein standard), and AVIDIN-60UL (avidin HRP-conjugate).
Human SCF ABTS ELISA Development Kit contains the key components required for the quantitative measurement of natural and/or recombinant human SCF in a sandwich ELISA format. Human SCF ABTS ELISA Development Kit contains enough material to assay the target cytokine in at least 1,000 ELISA plate wells.
The ABTS ELISA Buffer Kit (Product # 900-K00), sold separately, is recommended to be used in conjunction with this ABTS ELISA Development Kit for optimal performance. This buffer kit contains all the necessary components to assay ten 96-well ELISA plates (included) and contains detailed handling instructions.
Target information
Stem cell factor (SCF) is the ligand of the c-Kit oncogene and is expressed by various structural and inflammatory cells in the airways. Binding of SCF by the c-Kit receptor leads to homodimerization of the receptor and the activation of signalling pathways such as PI-3, PLC-gamma, Jak/STAT, and MAP kinase pathways. SCF expression leads to the induction of mast cell survival and the expression and release of histamine, pro-inflammatory cytokines and chemokines. The inhibition of the SCF/c-Kit pathway leads to a decrease in histamine levels, mast cell and eosinophil infiltration, IL-4 production and airway hyperresponsiveness, suggesting this pathway may be a useful therapeutic target in inflammatory diseases such as asthma. At least two isoforms of SCF are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : DCUA, DFNA69, FPH2, FPHH, Kitl, KITLG, KL-1, MGF, SCF, SF, SHEP7
Gene ID : (Human) 4254
Gene symbol : KITLG
Protein Aliases : c-Kit ligand, familial progressive hyperpigmentation 2, Kit ligand, Mast cell growth factor, MGF, SCF, steel factor, Stem cell factor
UniProt ID (Human) P21583
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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