Invitrogen

Human SMAD1 (Phospho) [pS463/pS465] InstantOne™ ELISA Kit

View all (2) SMAD1 ELISA Kits
Technical guide & protocol Tech Support
Technical guide & protocol Tech Support

Product Specifications

Sample type/volume

Cell Lysate
20 µL

Hands-on time

1 hr 20 min

Time-to-result

1 hr 10 min

Homogenous (no wash)

No

Instrument

Colorimetric Microplate Reader

Product size

96 Tests

Contents

Capture Antibody
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal

Shipping conditions

Wet ice

Storage

2-8°C

Protein name

SMAD1

Target site-specificity

Phosphorylated Ser463, Phosphorylated Ser465

Species (tested)

Human

Assay kit format

InstantOne ELISA

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated human SMAD1 (Ser463/465) in cell lysates. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.

SMAD1 is a member of a family of transcription factors involved in mediating signaling by growth and differentiation factors such as transforming growth factor beta (TGF-b) and bone morphogenic protein (BMP).

Target information

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : BSP-1, BSP1, JV4-1, JV41, MADH1, MADR1, SMAD1

Gene ID : (Human) 4086

Gene symbol : SMAD1

Protein Aliases : BSP-1, JV4-1, MAD homolog 1, MAD, mothers against decapentaplegic homolog 1, Mad-related protein 1, Mothers against decapentaplegic homolog 1, mothers against DPP homolog 1, SMAD 1, SMAD family member 1, SMAD, TGF-beta signaling protein 1, transforming growth factor-beta signaling protein 1, Transforming growth factor-beta-signaling protein 1

UniProt ID (Human) Q15797

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