Invitrogen

Human SMAD3 (Phospho) [pS423/pS425] InstantOne™ ELISA Kit

Technical guide & protocol Tech Support
Technical guide & protocol Tech Support

Product Specifications

Sample type/volume

Cell Lysate
20 µL

Hands-on time

1 hr 20 min

Time-to-result

1 hr 10 min

Homogenous (no wash)

No

Instrument

Colorimetric Microplate Reader

Product size

96 Tests

Contents

Capture Antibody
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal

Shipping conditions

Wet ice

Storage

2-8°C

Protein name

SMAD3

Target site-specificity

Phosphorylated Ser423, Phosphorylated Ser425

Species (tested)

Human

Assay kit format

InstantOne ELISA

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated human SMAD3 (Ser423/425) in cell lysates. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.

Suitable Sample Types

Tested on cell culture samples. Expected to work on many other sample types, but have not been tested at this time.

Target information

SMAD3 belongs to the SMAD family of proteins. They are similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : HSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3, SMAD3

Gene ID : (Human) 4088

Gene symbol : SMAD3

Protein Aliases : hMAD-3, hSMAD3, JV15-2, MAD homolog 3, mad homolog JV15-2, mad protein homolog, MAD, mothers against decapentaplegic homolog 3, mad3, Mothers against decapentaplegic homolog 3, mothers against DPP homolog 3, SMA- and MAD-related protein 3, SMAD 3, SMAD family member 3, SMAD

UniProt ID (Human) P84022

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