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Human STAT3 (Total) InstantOne™ ELISA Kit
Representative data for STAT3 (Total) Multispecies InstantOne™ ELISA. A431 cells were pre-treated with 10 µM AG1478 (an EGFR kinase inhibitor), washed and unstimulated (-) or stimulated with 10 ng/mL EGF for 10 min (+). Cells were analyzed by immunoblotting (left) and InstantOne™ ELISA (right). The intensity of the bands around 89 kDa as detected by immunoblot correlates with InstantOne™ ELISA absorption values.
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Product Specifications
Sample type/volume
Cell Lysate
20 µL
Hands-on time
1 hr 20 min
Time-to-result
1 hr 10 min
Homogenous (no wash)
No
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Capture Antibody
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
STAT3
Target sub-specificity
Total
Species (tested)
Human
Assay kit format
InstantOne ELISA
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The InstantOne™ ELISA is specifically engineered for accurate measurement of total human STAT3 in cell lysates. Detection of mouse and rat STAT3 is expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.
Target information
STAT3 belongs to the family of STAT (signal transducers and activators of transcription) proteins which are phosphorylated by receptor associated kinases, translocate to the nucleus, and act as transcription factors in response to cytokines and growth factors. Coactivators such as CREB-binding protein are required for the transcriptional activation by STAT3. STAT3 can also be activated by Interferon-alpha, Interferon-gamma, EGF, PDGF and IL6. Phosphorylation on tyrosine 705 by JAK1 and JAK2 is essential for STAT3 dimer formation, nuclear translocation, and DNA binding activity. In humans, the STAT3 gene is located on the q arm of chromosome 17. STAT3 has been shown to be activated by IFN-alpha but not IFN-beta. The transcription factors associated with STAT3 are c-Jun and cyclic AMP-responsive enhancer binding protein (CREB). STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3 while the PIAS3 protein is a specific inhibitor of STAT3. Three alternatively spliced transcript variants encoding distinct isoforms of STAT3 have been described. Deletion of the STAT3 gene in knock-out mice was lethal at the early embryonic stage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : ADMIO, ADMIO1, APRF, HIES, STAT3
Gene ID : (Human) 6774
Gene symbol : STAT3
Protein Aliases : Acute-phase response factor, DNA-binding protein APRF, Signal transducer and activator of transcription 3, signal transducer and activator of transcription 3 (acute-phase response factor)
UniProt ID (Human) P40763
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