Invitrogen

Human STAT5 alpha (Total/Phospho) [pY694/pY699] InstantOne™ ELISA Kit

Technical guide & protocol Tech Support
Technical guide & protocol Tech Support

Product Specifications

Sample type/volume

Cell Lysate
20 µL

Hands-on time

1 hr 20 min

Time-to-result

1 hr 10 min

Homogenous (no wash)

No

Instrument

Colorimetric Microplate Reader

Product size

96 Tests

Contents

Capture Antibody
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal

Shipping conditions

Wet ice

Storage

2-8°C

Protein name

STAT5 alpha

Target site-specificity

Phosphorylated Tyr694, Phosphorylated Tyr699

Target sub-specificity

Total

Species (tested)

Human

Assay kit format

InstantOne ELISA

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

The InstantOne™ ELISA is specifically engineered for accurate measurement of total and phosphorylated human STAT5A/B (Tyr694/Tyr699) in cell lysates. Detection of mouse and rat STAT5A/B is expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.

Target information

STAT5 alpha is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5 alpha is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of STAT5 alpha in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of STAT5 alpha is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this protein in cells. STAT5 alpha, along with STAT5 beta, were identified in mouse. The amino acid sequences of STAT5 alpha and STAT5 beta show 96% sequence similarity, and both proteins are co expressed in most tissues in virgin and lactating mice. However, differential accumulation of STAT5 alpha and STAT5 beta mRNA has been reported for both muscle and mammary tissue. STAT5 alpha is critically involved in a variety of physiological functions, including reproduction, lactation, immune function and somatic growth.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?
Let us know so we can reference it here.
Cite this product

Bioinformatics

Gene aliases : MGF, STAT5, STAT5A

Gene ID : (Human) 6776

Gene symbol : STAT5A

Protein Aliases : Signal transducer and activator of transcription 5A

UniProt ID (Human) P42229

guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support