Technical guide & protocol Tech Support

Product Specifications

Analytical sensitivity

0.62 ng/mL

Assay range

0.62-150 ng/mL

Sample type/volume

Serum
50 µL
Supernatant
50 µL
Plasma
50 µL

Hands-on time

1 hr 20 min

Time-to-result

4 hr 45 min

Homogenous (no wash)

No

Interassay CV

<12%

Intraassay CV

<10%

Instrument

Colorimetric Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

2-8°C

Protein name

CD100 (SEMA4D)

Species (tested)

Human

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

Human Semaphorin 4D quantitates human Semaphorin 4D in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Semaphorin 4D.

Target information

Semaphorins are neuronal chemorepellants that direct pioneering neurons during nervous system development. CD100 (also known as semaphorin 4d), a 150 kDa homodimer, is a novel semaphorin that induces B cells to aggregate and improves their viability in vitro. CD100 modifies CD40-CD40L B cell signaling by augmenting B cell aggregation and survival and down regulating CD23 expression. CD72 is a lymphocyte receptor for the class IV semaphorin CD100, which is involved in regulating B cell signaling. CD100 is expressed on resting and PHA stimulated T cells. The protein is weakly expressed on NK cells, EBV transformed B cells, monocytes and tumor/peripheral blood T cell lines, and at higher density on activated T cells and B cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : C9orf164, CD100, coll-4, M-sema-G, SEMA4D, SEMAJ

Gene ID : (Human) 10507

Gene symbol : SEMA4D

Protein Aliases : A8, BB18, CD100, GR3, sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D, 4D, Semaphorin-4D

UniProt ID (Human) Q92854

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