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![Human Smad2 [pSpS465/467] ELISA™ Kit (KHO8011)](https://tfcom-global-nginx.commerceprod.thermofisher.com/antibody/images/650/Sandwich-Direct-ELISA-Kit-Packaging-650x600.jpg?time=20240507 "Human Smad2 [pSpS465/467] ELISA™ Kit (KHO8011)")
![Human Smad2 [pSpS465/467] ELISA™ Kit (KHO8011)](https://tfcom-global-nginx.commerceprod.thermofisher.com/antibody/images/150/Sandwich-Direct-ELISA-Kit-Packaging-650x600.jpg?time=20240507)
Product Specifications
Analytical sensitivity
0.42 U/mL
Assay range
0-10 U/mL
Sample type/volume
Cell Lysate
10 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 30 min
Homogenous (no wash)
No
Interassay CV
6.6%
Intraassay CV
3.9%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard (2 vials)
Assay Buffer concentrate
Biotinylated Detection Antibody
Streptavidin-HRP
Wash Buffer
Substrate Solution
Stop Solution
Adhesive Plate Covers
Standard (2 vials)
Assay Buffer concentrate
Biotinylated Detection Antibody
Streptavidin-HRP
Wash Buffer
Substrate Solution
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
SMAD2
Target sub-specificity
Multisubtype
Protein family
Cell Signaling
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
HRP
Label or dye
HRP
About This Kit
The Human Smad2 ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of human Smad2.
Target information
SMAD2 (MADH2, MAD2) regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. SMAD2 interacts with the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation into the nucleus is a central event in TGF beta signaling. Phosphorylation of threonine 8 in the calmodulin-binding region of the MH1 domain by extracellular signal regulated kinase 1 (ERK1) enhances SMAD2 transcriptional activity, which is negatively regulated by calmodulin. In response to the TGF-beta signal, SMAD2 is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. SMAD2 can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the SMAD2 protein have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : hMAD-2, hSMAD2, JV18, JV18-1, MADH2, MADR2, SMAD2
Gene ID : (Human) 4087
Gene symbol : SMAD2
Protein Aliases : hMAD-2, JV18-1, MAD homolog 2, Mad-related protein 2, mother against DPP homolog 2, Mothers against decapentaplegic homolog 2, Sma- and Mad-related protein 2, SMAD 2, SMAD family member 2, SMAD, mothers against DPP homolog 2
UniProt ID (Human) Q15796
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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