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Product Specifications
Analytical sensitivity
Lot-specific
Assay range
Lot-specific
Hands-on time
1 hr 45 min
Time-to-result
24 hr 30 min
Homogenous (no wash)
No
Instrument
Colorimetric Microplate Reader
Product size
10 x 96 Tests
Contents
This Human TNF alpha ELISA Kit includes Capture Antibody, Detection Antibody, Protein Standard, and HRP-conjugate. The HRP-conjugate is packaged separately.
Storage
Refer to product documentation for component specific storage temperature.
Protein name
TNF alpha
Protein family
Growth Factors & Receptors
Species (tested)
Human
Assay kit format
ELISA Development Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
900-K25K consists of: 900-K25 (capture antibody, detection antibody, and protein standard), and AVIDIN-60UL (avidin HRP-conjugate).
Human TNF alpha ABTS ELISA Development Kit contains the key components required for the quantitative measurement of natural and/or recombinant human TNF alpha in a sandwich ELISA format. Human TNF alpha ABTS ELISA Development Kit contains enough material to assay the target cytokine in at least 1,000 ELISA plate wells.
The ABTS ELISA Buffer Kit (Product # 900-K00), sold separately, is recommended to be used in conjunction with this ABTS ELISA Development Kit for optimal performance. This buffer kit contains all the necessary components to assay ten 96-well ELISA plates (included) and contains detailed handling instructions.
Target information
TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : DIF, TNF, TNF-alpha, TNFA, TNFSF2, TNLG1F
Gene ID : (Human) 7124
Gene symbol : TNF
Protein Aliases : APC1 protein, Cachectin, TNF, macrophage-derived, monocyte-derived, TNF-a, TNF-alpha, Tumor necrosis factor, tumor necrosis factor ligand 1F, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
UniProt ID (Human) P01375
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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