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Product Specifications
Analytical sensitivity
Lot-specific
Assay range
Lot-specific
Hands-on time
1 hr 45 min
Time-to-result
24 hr 30 min
Homogenous (no wash)
No
Instrument
Colorimetric Microplate Reader
Product size
10 x 96 Tests
Contents
This Human TRAIL (CD253) ELISA Kit includes Capture Antibody, Detection Antibody, Protein Standard, and HRP-conjugate. The HRP-conjugate is packaged separately.
Storage
Refer to product documentation for component specific storage temperature.
Protein name
TRAIL (CD253)
Protein family
Growth Factors & Receptors
Species (tested)
Human
Assay kit format
ELISA Development Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
900-K141K consists of: 900-K141 (capture antibody, detection antibody, and protein standard), and AVIDIN-60UL (avidin HRP-conjugate).
Human TRAIL (CD253) ABTS ELISA Development Kit contains the key components required for the quantitative measurement of natural and/or recombinant human TRAIL (CD253) in a sandwich ELISA format. Human TRAIL (CD253) ABTS ELISA Development Kit contains enough material to assay the target cytokine in at least 1,000 ELISA plate wells.
The ABTS ELISA Buffer Kit (Product # 900-K00), sold separately, is recommended to be used in conjunction with this ABTS ELISA Development Kit for optimal performance. This buffer kit contains all the necessary components to assay ten 96-well ELISA plates (included) and contains detailed handling instructions.
Target information
TRAIL is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. TRAIL preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. TRAIL binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of TRAIL protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3. Receptors for TRAIL include two death domain containing receptors, death receptor 4 (DR4) and DR5, as well as two decoy receptors, DcR1 and DcR2, lacking the intracellular signaling death domain. TRAIL is a type II membrane protein and expressed in a variety of human tissues. Like TNF and Fas ligand, TRAIL induces apoptosis and NF-kB activation in many tissues and cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : Apo-2L, APO2L, CD253, TL2, TNFSF10, TNLG6A, TRAIL
Gene ID : (Human) 8743
Gene symbol : TNFSF10
Protein Aliases : Apo-2 ligand, Apo-2L, CD253, chemokine tumor necrosis factor ligand superfamily member 10, Protein TRAIL, TNF-related apoptosis inducing ligand TRAIL, TNF-related apoptosis-inducing ligand, tumor necrosis factor (ligand) family, member 10, tumor necrosis factor (ligand) superfamily member 10, tumor necrosis factor (ligand) superfamily, tumor necrosis factor apoptosis-inducing ligand splice variant delta, tumor necrosis factor ligand 6A, Tumor necrosis factor ligand superfamily member 10
UniProt ID (Human) P50591
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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