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Product Specifications

Analytical sensitivity

18.75 pg/mL

Assay range

31.25-2000 pg/mL

Sample type/volume

Tissue Homogenate
100 µL
Plasma
100 µL
Serum
100 µL
Cell Lysate
100 µL

Hands-on time

1 hr 20 min

Time-to-result

2 hr 30 min

Homogenous (no wash)

No

Interassay CV

<10%

Intraassay CV

<10%

Instrument

Colorimetric Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer

Shipping conditions

Wet ice

Storage

Refer to product documentation for component specific storage temperature.

Protein name

Tau

Species (reported)

Human

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

Unconjugated

About This Kit

The Human phosphorylated microtubule associated protein tau (pMAPT/pTAU) ELISA quantitates pMAPT/pTAU in serum, plasma and other biological fluids.

Principle of the method

The Human pMAPT/pTAU solid phase sandwich ELISA (enzyme linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target specific antibody has been pre coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme antibody target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Rigorous validation:

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Target information

Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerization and stabilize microtubules. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton while the longer isoforms may preferentially play a role in its stabilization. In its hyper-phosphorylated form, Tau is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer's diseases (AD) brain. Hyper-phosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases phosphorylate Tau, including GSK-3beta, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II. Hyper-phosphorylated Tau is found in neurofibrillary lesions in a range and other central nervous system disorders such as Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : DDPAC, FTDP-17, MAPT, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU

Gene ID : (Human) 4137

Gene symbol : MAPT

Protein Aliases : G protein beta1/gamma2 subunit-interacting factor 1, Microtubule-associated protein tau, microtubule-associated protein tau, isoform 4, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, protein phosphatase 1, regulatory subunit 103

UniProt ID (Human) P10636

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