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Product Specifications

Analytical sensitivity

4 pg/mL

Assay range

2.744-2000 pg/mL

Sample type/volume

Plasma
50 µL
Serum
50 µL
Supernatant
100 µL

Hands-on time

1 hr 20 min

Time-to-result

4 hr 45 min

Homogenous (no wash)

No

Interassay CV

<12%

Intraassay CV

<10%

Instrument

Colorimetric Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

2-8°C

Protein name

Thyroid Stimulating Hormone

Protein family

Hormones & Receptors

Species (tested)

Human

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

The Human TSH (CGA) ELISA quantitates Hu TSH in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TSH.

Principle of the method

The Human TSH solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Target information

The four human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. The protein encoded by this gene is the alpha subunit and belongs to the glycoprotein hormones alpha chain family.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : CG-ALPHA, CGA, FSHA, GPHa, GPHA1, HCG, LHA, TSH-B, TSH-BETA, TSHA, TSHB

Gene ID : (Human) 1081, (Human) 7252

Gene symbol : CGA, TSHB

Protein Aliases : Anterior pituitary glycoprotein hormones common subunit alpha, CG-alpha, Choriogonadotropin alpha chain, Chorionic gonadotrophin subunit alpha, chorionic gonadotropin, alpha polypeptide, Follicle-stimulating hormone alpha chain, follicle-stimulating hormone alpha subunit, Follitropin alpha chain, FSH-alpha, Glycoprotein hormones alpha chain, LSH-alpha, Luteinizing hormone alpha chain, Lutropin alpha chain, thyroid stimulating hormone, beta, Thyroid-stimulating hormone alpha chain, Thyroid-stimulating hormone subunit beta, Thyrotropin alpha chain, Thyrotropin beta chain, Thyrotropin subunit beta, TSH-alpha, TSH-B

UniProt ID (Human) P01215, (Human) P01222

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