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Product Specifications
Analytical sensitivity
19 pg/mL
Assay range
31.3-2,000 pg/mL
Sample type/volume
Amniotic Fluid
10 µL
Plasma
10 µL
Serum
10 µL
Supernatant
10 µL
Hands-on time
1 hr 20 min
Time-to-result
3 hr 10 min
Homogenous (no wash)
No
Interassay CV
4.5%
Intraassay CV
3%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Controls
Adhesive Plate Covers
Standard
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Controls
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
AOC3
Target sub-specificity
Soluble
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Human Vascular Adhesion Protein (Hu VAP-1) ELISA quantitates Hu VAP-1 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu VAP-1.
Principle of the method
The Human VAP-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
Copper amine oxidases catalyze the oxidative conversion of amines to aldehydes in the presence of copper and quinone cofactor. The product is a major protein on the adipocyte plasma membrane. It has adhesive properties and also has functional monoamine oxidase activity. A pseudogene for this gene has been discribed and is located approximately 9-kb downstream.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : AOC3, HPAO, SSAO, VAP-1, VAP1
Gene ID : (Human) 8639
Gene symbol : AOC3
Protein Aliases : Amine oxidase [copper-containing] 3, Amine oxidase copper-containing 3, amine oxidase, copper containing 3 (vascular adhesion protein 1), Copper amine oxidase, HPAO, placenta copper monamine oxidase, Semicarbazide-sensitive amine oxidase, SSAO, VAP-1, Vascular adhesion protein 1
UniProt ID (Human) Q16853
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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