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Product Specifications
Analytical sensitivity
0.4 ng/mL
Assay range
0.4-100 ng/mL
Sample type/volume
Supernatant
50 µL
Plasma
50 µL
Serum
50 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 45 min
Homogenous (no wash)
No
Interassay CV
<12%
Intraassay CV
<10%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
eNOS
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
Human eNOS/NOS3 quantitates human eNOS/NOS3 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human eNOS/NOS3.
Target information
Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : ECNOS, eNOS, NOS3
Gene ID : (Human) 4846
Gene symbol : NOS3
Protein Aliases : cNOS, Constitutive NOS, EC-NOS, Endothelial NOS, Nitric oxide synthase 3, nitric oxide synthase 3 (endothelial cell), nitric oxide synthase 3 transcript variant eNOS-delta20, nitric oxide synthase 3 transcript variant eNOS-delta20-21, nitric oxide synthase 3 transcript variant eNOS-delta21, Nitric oxide synthase, endothelial, NOS type III, NOSIII
UniProt ID (Human) P29474
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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