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Product Specifications

Analytical sensitivity

0.059 U/mL

Assay range

0.09-6.0 U/mL

Sample type/volume

Plasma
10 µL
Serum
10 µL
Supernatant
10 µL

Hands-on time

15 min

Time-to-result

2 hr 10 min

Homogenous (no wash)

No

Interassay CV

<10%

Intraassay CV

<5%

Instrument

Colorimetric Microplate Reader

Product size

128 Tests

Contents

Pre-coated 96 well Microplate
Detection Antibody
Anti-IgG-HRP
Sample Diluent, lyophilized
Standard (8-well strips x 4)
Wash Buffer Concentrate
Substrate Solution
Sample Diluent
Stop Solution
Lysis Buffer 10x
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

-20°C

Protein name

TNFR1

Target sub-specificity

Soluble

Species (tested)

Baboon, Cynomolgus monkey

Assay kit format

Instant ELISA Kit

Detector antibody conjugate

HRP

Label or dye

HRP

About This Kit

The Monkey Soluble Tumor Necrosis Factor Receptor 1(Mk sTNRF1) ELISA quantitates Mk sTNFR1 in monkey serum, plasma, cell culture supernatants, or other body fluids. The assay will exclusively recognize both natural and recombinant Mk sTNFR1.

Principle of the method

The Monkey sTNFR1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Target information

TNFR1 (Tumor necrosis factor receptor 1) belongs to the tumor necrosis factor superfamily, and is one of the major TNF-alpha receptors. TNFR1 plays an important role in mediating, in cytokine mediated signaling, positive regulation of the NF-Kb pathway, positive regulation of angiogenesis, and negative regulation of gene expression. The extracellular domain of TNFR1 is also released into the circulatory system as soluble TNFR1 (sTNFR1). In humans, the TNFR1 gene is located on chromosome 12. Anti-apoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with TNFR1, and thus play regulatory roles in the signal transduction mediated by the receptor. Germline mutations of the extracellular domains of TNFR1 were found to be associated with the autosomal dominant periodic fever syndrome, and the impaired receptor clearance is thought to be a mechanism of the disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene ID : (Cynomolgus monkey) 102135285

Gene symbol : TNFRSF1A

Protein Aliases : tumor necrosis factor receptor superfamily, member 1A

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