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Product Specifications
Analytical sensitivity
37.5 pg/mL
Assay range
62.5-4000 pg/mL
Sample type/volume
Serum
100 µL
Plasma
100 µL
Hands-on time
1 hr 20 min
Time-to-result
3 hr 30 min
Homogenous (no wash)
No
Interassay CV
<10%
Intraassay CV
<10%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Shipping conditions
Wet ice
Storage
Refer to product documentation for component specific storage temperature.
Protein name
BAFF (BLyS)
Protein family
Tumor necrosis factor ligand
Species (tested)
Mouse
Assay kit format
Sandwich ELISA Kit
Label or dye
HRP
About This Kit
The Mouse BAFF/CD257( B-cell Activating Factor) ELISA quantitates BAFF in serum, plasma, and other biological fluids
The Mouse BAFF/CD257( B-cell Activating Factor) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
BAFF is a membrane bound cytokine expressed by B cell lineage cells. The soluble form of the cytokine binds to the receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. BAFF is a potent B cell activator, and important for the proliferation and differentiation of B cells. BAFF is also expressed by T lymphocytes and has been shown to promote T cell activation and survival. Isoforms representing alternatively spliced, and glycosylated variants have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : BAFF, BLyS, D8Ertd387e, TALL-1, TALL1, THANK, Tnfsf13b, TNFSF20, zTNF4
Gene ID : (Mouse) 24099
Gene symbol : Tnfsf13b
Protein Aliases : b cell-activating factor, B-cell-activating factor, BAFF, CD257, Tumor necrosis factor ligand superfamily member 13B
UniProt ID (Mouse) Q9WU72
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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