Technical guide & protocol Tech Support

Product Specifications

Analytical sensitivity

0.41 ng/mL

Assay range

0.41-100 ng/mL

Sample type/volume

Serum
50 µL
Supernatant
50 µL
Plasma
50 µL

Hands-on time

1 hr 20 min

Time-to-result

4 hr 45 min

Homogenous (no wash)

No

Interassay CV

<12%

Intraassay CV

<10%

Instrument

Colorimetric Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

2-8°C

Protein name

BCL-W

Species (tested)

Mouse

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

Mouse BCL-W quantitates mouse BCL-W in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant mouse BCL-W.

Target information

Using a PCR-based strategy, an additional protein with life-promoting activity, designated Bcl-w, has been identified. The protein is highly conserved between mouse and human and is encoded by a gene located near the TCRalpha gene on chromosome 14. Bcl-w is expressed in myeloid cell lines but not in T and B lymphocytes, and can be found in a wide range of tissues. An alternative splicing event in exon 4 results in two transcripts. The first, Bcl-w, encodes a protein of 193 amino acids, and the second, Bcl-w/rox, encodes a protein 333 amino acids in length. The "rox" portion of Bcl-w/rox shows a striking 66% amino acid sequence identity with the Drosophila rox2 protein; however, the Bcl-w/rox transcript may be expressed at very low levels.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : AW048834, Bcl-w, Bcl2l2, Bclw, c98, Gtrgal2, Gtrosa41, Kiaa0271

Gene ID : (Mouse) 12050

Gene symbol : Bcl2l2

Protein Aliases : Apoptosis regulator Bcl-W, Bcl-2-like protein 2, Bcl2-L-2, c98

UniProt ID (Mouse) P70345

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