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Product Specifications
Analytical sensitivity
2 pg/mL
Assay range
1.37-1000 pg/mL
Sample type/volume
Plasma
5 µL
Serum
5 µL
Supernatant
100 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 45 min
Homogenous (no wash)
No
Interassay CV
<12%
Intraassay CV
<10%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
CXCL13
Protein family
Chemokines & Receptors
Species (tested)
Mouse
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Mouse BLC/CXCL13 ELISA quantitates Ms BLC in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms BLC.
Principle of the method
The Mouse BLC solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
CXCL13, also known as BCA-1 and Angie, is a small chemokine strongly expressed in the secondary lymphoid organs. It preferentially promotes the migration of B lymphocytes (both B-1 and B-2) to the follicles of the spleen, lymph nodes, and Peyer's patches. It is produced by macrophages, and in mouse the highest constitutive producers include large peritoneal macrophages, Kuppfer cells and lung macrophages (both CD11c+ and CD11c-). The cellular ligand for CXCL13 is CXCR5 also known as BLR-1 (Burkitt’s Lymphoma Receptor 1). CXCR5 is expressed on B cells, Tfh cells and, to a lower degree, some memory T cell populations. CXCL13 plays an important role in the development of chronic lymphocytic leukemia (CCL). CXCL13 is also a plasma biomarker for germinal center activity and generation of broadly neutralizing antibodies against HIV.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Gene aliases : 4631412M08Rik, Angie, ANGIE2, BCA-1, BLC, BLR1L, Cxcl13, Scyb13
Gene ID : (Mouse) 55985
Gene symbol : Cxcl13
Protein Aliases : B lymphocyte chemoattractant, CXC chemokine BLC, Small-inducible cytokine B13, C-X-C motif chemokine 13, small inducible cytokine subfamily B (Cys-X-Cys), member 13
UniProt ID (Mouse) O55038
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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