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Product Specifications
Analytical sensitivity
0.94 ng/mL
Assay range
1.56-100 ng/mL
Sample type/volume
Plasma
100 µL
Serum
100 µL
Hands-on time
1 hr 20 min
Time-to-result
3 hr 30 min
Homogenous (no wash)
No
Interassay CV
<10%
Intraassay CV
<10%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Shipping conditions
Wet ice
Storage
Refer to product documentation for component specific storage temperature.
Protein name
Follicle Stimulating Hormone
Protein family
Glycoprotein hormone
Species (tested)
Mouse
Assay kit format
Sandwich ELISA Kit
Label or dye
HRP
About This Kit
The Mouse FSH (Follicle Stimulating Hormone) ELISA quantitates FSH in serum, plasma, and other biological fluids
The Mouse FSH (Follicle Stimulating Hormone) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
Follicle-stimulatine hormone (FSH) is a member of the glycoprotein hormone family. Members of this family consist of an identical alpha subunit and a hormone-specific beta subunit. Alternative splicing results in two transcript variants encoding the same protein. FSH is secreted by gonadotropic cells in the pituitary gland and in conjunction with luteinizing hormone, induces egg and sperm production.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : FSH, Fshb, Fshbeta
Gene ID : (Mouse) 14308
Gene symbol : Fshb
Protein Aliases : Follicle-stimulating hormone beta subunit, Follitropin beta chain, Follitropin subunit beta, FSH-B, FSH-beta
UniProt ID (Mouse) Q60687
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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