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Product Specifications
Analytical sensitivity
12 pg/mL
Assay range
16.38-4000 pg/mL
Sample type/volume
Plasma
1 µL
Serum
1 µL
Supernatant
100 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 45 min
Homogenous (no wash)
No
Interassay CV
<12%
Intraassay CV
<10%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
Galectin 1
Protein family
Other Proteins
Species (tested)
Mouse
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Mouse Galectin-1 (LGALS1) ELISA quantitates Ms Galectin-1 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms Galectin-1.
Principle of the method
The Mouse Galectin-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
Galectin-1 belongs to a large family of carbohydrate-binding proteins called lectins. Galectin-1can be either monomeric or homodimeric and is found in a wide variety of cells and tissue types. Galectin-1 can control cell growth, proliferation, induce apoptosis of activated T cells while it can also modulate cytokine secretion or inhibit pro-inflammatory cytokine production. Galectin-1 plays an important role in acute and chronic inflammation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : AA410090, Gal-1, Galbp, galectin-1, Gbp, L-14.5, L14, Lect14, Lgals1
Gene ID : (Mouse) 16852
Gene symbol : Lgals1
Protein Aliases : 14 kDa lectin, beta-galactoside binding protein, Beta-galactoside-binding lectin L-14-I, Gal-1, Galaptin, Galectin-1, Lactose-binding lectin 1, Lectin galactoside-binding soluble 1, S-Lac lectin 1
UniProt ID (Mouse) P16045
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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