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Product Specifications
Analytical sensitivity
6.5 pg/mL
Assay range
7.8-500 pg/mL
Sample type/volume
Plasma
50 µL
Serum
50 µL
Supernatant
50 µL
Hands-on time
1 hr 20 min
Time-to-result
3 hr 10 min
Homogenous (no wash)
No
Interassay CV
7.2%
Intraassay CV
8.4%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
CXCL10 (IP-10)
Species (tested)
Mouse
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Mouse Inflammatory Protein 10/CXCL10 (Ms IP-10) ELISA quantitates Ms IP-10 in mouse serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IP-10.
Principle of the method
The Mouse IP-10 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
IP-10 (interferon-gamma induced protein 10 kD; in mouse, also called cytokine responsive gene 2, Crg-2, or mob-1) is a highly inducible, primary response gene that belongs to the CXC chemokine superfamily. It was first cloned in 1985. The biological functions of IP-10 are still unclear. Like Mig, IP-10 has no activity on neutrophils. Its functions include stimulation of monocytes, natural killer and T-cell migration, regulation of T-cell and bone marrow progenitor maturation, modulation of adhesion molecule expression as well as inhibition of angiogenesis. Mig and IP-10 share the same receptor, CXCR3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Gene aliases : C7, CRG-2, Crg2, Cxcl10, gIP-10, Ifi10, INP10, IP-10, IP10, mob-1, Scyb10
Gene ID : (Mouse) 15945
Gene symbol : Cxcl10
Protein Aliases : 10 kDa interferon gamma-induced protein, C-X-C motif chemokine 10, C7, Gamma-IP10, interferon activated gene 10, Interferon-gamma induced protein CRG-2, interferon-gamma-induced protein CRG-2, small inducible cytokine B subfamily (Cys-X-Cys), member 10, Small-inducible cytokine B10
UniProt ID (Mouse) P17515
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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