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Product Specifications
Analytical sensitivity
7.8 pg/mL
Assay range
7.8-500 pg/mL
Sample type/volume
Plasma
50 µL
Serum
50 µL
Supernatant
50 µL
Hands-on time
24 hr
Time-to-result
3 hr 10 min
Homogenous (no wash)
No
Instrument
Colorimetric Microplate Reader
Product size
10 x 96 Tests
Contents
Capture (coating) Antibody
Detection Antibody
Standard
SAV-HRP
Sample Diluent
Detection Antibody
Standard
SAV-HRP
Sample Diluent
Shipping conditions
Wet ice
Storage
-20°C
Protein name
CXCL10 (IP-10)
Species (tested)
Mouse
Assay kit format
Matched Antibody Pair
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Mouse IP-10 Matched Antibody Pair Kit comes with pre-matched antibody pairs, standards, sample dilutent and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse IP-10. Buffer reagents and microplates needed to complete the ELISA reaction are sold separately.
Principle of the method
ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Target information
IP-10 (interferon-gamma induced protein 10 kD; in mouse, also called cytokine responsive gene 2, Crg-2, or mob-1) is a highly inducible, primary response gene that belongs to the CXC chemokine superfamily. It was first cloned in 1985. The biological functions of IP-10 are still unclear. Like Mig, IP-10 has no activity on neutrophils. Its functions include stimulation of monocytes, natural killer and T-cell migration, regulation of T-cell and bone marrow progenitor maturation, modulation of adhesion molecule expression as well as inhibition of angiogenesis. Mig and IP-10 share the same receptor, CXCR3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : C7, CRG-2, Crg2, Cxcl10, gIP-10, Ifi10, INP10, IP-10, IP10, mob-1, Scyb10
Gene ID : (Mouse) 15945
Gene symbol : Cxcl10
Protein Aliases : 10 kDa interferon gamma-induced protein, C-X-C motif chemokine 10, C7, Gamma-IP10, interferon activated gene 10, Interferon-gamma induced protein CRG-2, interferon-gamma-induced protein CRG-2, small inducible cytokine B subfamily (Cys-X-Cys), member 10, Small-inducible cytokine B10
UniProt ID (Mouse) P17515
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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