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Product Specifications
Analytical sensitivity
2.6 pg/mL
Assay range
15.6-1,000 pg/mL
Sample type/volume
Plasma
50 µL
Serum
50 µL
Supernatant
50 µL
Hands-on time
15 min
Time-to-result
3 hr 10 min
Homogenous (no wash)
No
Interassay CV
9.5%
Intraassay CV
7.7%
Instrument
Colorimetric Microplate Reader
Product size
128 Tests
Contents
Pre-coated 96 well Microplate
Biotinylated Detection Antibody
SAV-HRP
Sample Diluent, lyophilized
Standard (8-well strips x 4)
Wash Buffer Concentrate
Substrate Solution
Sample Diluent
Stop Solution
Lysis Buffer 10x
Adhesive Plate Covers
Biotinylated Detection Antibody
SAV-HRP
Sample Diluent, lyophilized
Standard (8-well strips x 4)
Wash Buffer Concentrate
Substrate Solution
Sample Diluent
Stop Solution
Lysis Buffer 10x
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
-20°C
Protein name
CCL7 (MCP3)
Species (tested)
Mouse
Assay kit format
Instant ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Mouse Moncyte Chemoattractant Protein 3 (Ms MCP-3) ELISA quantitates Ms MCP-3 in mouse serum, plasma, cell culture supernatants or other body fluids. The assay will exclusively recognize both natural and recombinant Ms MCP-3.
Principle of the method
The Mouse MCP-3 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
This is a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Gene aliases : Ccl7, Fic, marc, MCP-3, Mcp3, Scya7
Gene ID : (Mouse) 20306
Gene symbol : Ccl7
Protein Aliases : C-C motif chemokine 7, Intercrine/chemokine MARC, MCP-3, Monocyte chemoattractant protein 3, Monocyte chemotactic protein 3, Protein FIC, RANTES/sis homolog, small inducible cytokine A7, Small-inducible cytokine A7
UniProt ID (Mouse) Q03366
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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