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Product Specifications
Analytical sensitivity
120 pg/mL
Assay range
120-15,000 pg/mL
Sample type/volume
Serum
100 µL
Supernatant
100 µL
Hands-on time
1 hr 45 min
Time-to-result
24 hr 30 min
Homogenous (no wash)
No
Instrument
Colorimetric Microplate Reader
Product size
10 x 96 Tests
Contents
Capture Antibody: Pre-titrated, purified antibody
Detection Antibody: Pre-titrated, biotin-conjugated antibody
Standard: Recombinant protein for generating standard curve and calibrating samples
Coating Buffer: 10X PBS ELISA Coating Buffer
Assay Diluent: 5X Concentrated
Detection Enzyme: Pre-titrated Avidin-HRP
Substrate Solution: Tetramethylbenzidine (TMB) Solution
Certificate of Analysis: Lot-specific instructions for the dilution of antibodies and standards
96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22 and -86)
Detection Antibody: Pre-titrated, biotin-conjugated antibody
Standard: Recombinant protein for generating standard curve and calibrating samples
Coating Buffer: 10X PBS ELISA Coating Buffer
Assay Diluent: 5X Concentrated
Detection Enzyme: Pre-titrated Avidin-HRP
Substrate Solution: Tetramethylbenzidine (TMB) Solution
Certificate of Analysis: Lot-specific instructions for the dilution of antibodies and standards
96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22 and -86)
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
MCPT-1
Species (tested)
Mouse
Assay kit format
Uncoated ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Mouse MCPT-1 (mMCP-1) Uncoated ELISA Kit contains pre-matched antibody pairs, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse MCPT-1. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.
Principle of the method
ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Target information
MCPT-1 is the only chymase expressed by intestinal mucosal mast cells, which are found in the intestinal epithelium. Although it is expressed constitutively and is detectable in the sera of normal mice, parasites in the gut cause systemic levels to increase dramatically within two days and peak at two weeks following infection. It plays an important role in host defense against intestinal parasites, as mice deficient in MCPT-1 display delayed response upon infection. Elevated MCPT-1 levels are also observed during intestinal allergic hypersensitivity reactions. The mechanism of its action is not fully understood, although it is believed to increase intestinal permeability similarly to rMCPT-II, possibly by cleaving the proteins at tight junctions between cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : AV080368, Mcp-1, Mcpt1
Gene ID : (Mouse) 17224
Gene symbol : Mcpt1
Protein Aliases : Mast cell protease 1, MMCP-1
UniProt ID (Mouse) P11034
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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