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Product Specifications
Analytical sensitivity
9.38 pg/mL
Assay range
15.63-1000 pg/mL
Sample type/volume
Plasma
50 µL
Serum
50 µL
Hands-on time
1 hr 20 min
Time-to-result
3 hr 30 min
Homogenous (no wash)
No
Interassay CV
<10%
Intraassay CV
<10%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Shipping conditions
Wet ice
Storage
Refer to product documentation for component specific storage temperature.
Protein name
BNP
Protein family
Natriuretic peptide
Species (tested)
Mouse
Assay kit format
Competitive ELISA
Label or dye
HRP
About This Kit
The Mouse Brain Natriuretic Peptide (BNP) ELISA quantitates BNP in serum, plasma and other biological fluids
Principle of the method:
The Competitive Brain Natriuretic Peptide (BNP) ELISA research-use-only kit is designed to quantitatively measure BNP in mice. A BNP standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to Mouse BNP is added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of Mouse BNP in the samples is then determined by comparing the OD of the samples to the standard curve.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
This gene is a member of the natriuretic peptide family and encodes a secreted protein which functions as a cardiac hormone. The protein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein's biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. A high concentration of this protein in the bloodstream is indicative of heart failure. Mutations in this gene have been associated with postmenopausal osteoporosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : AA408272, BNF, BNP, Nppb
Gene ID : (Mouse) 18158
Gene symbol : Nppb
Protein Aliases : Brain natriuretic factor prohormone, brain natriuretic peptide, Gamma-brain natriuretic peptide, Iso-ANP, natriuretic peptide precursor type B, Natriuretic peptides B, preproBNP, type-B natriuretic factor
UniProt ID (Mouse) P40753
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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