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Product Specifications
Analytical sensitivity
0.65 ng/mL
Assay range
0.65-150 ng/mL
Sample type/volume
Serum
50 µL
Plasma
50 µL
Supernatant
50 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 45 min
Homogenous (no wash)
No
Interassay CV
<12%
Intraassay CV
<10%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
Calprotectin
Species (tested)
Mouse
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
Mouse S100A8/S100A9 quantitates mouse S100A8/S100A9 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant mouse S100A8/S100A9.
Target information
The S100A8/A9 heterocomplex, also called MRP-8/MRP-14 complex or calprotectin, comprises 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages and ileal tissue eosinophils. Peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. The S100A8/A9 complex has antibacterial, antifungal, immunomodulating and antiproliferative effects. Besides this it is a potent chemotactic factor for neutrophils. Plasma concentrations are elevated in diseases associated with increased neutrophil activity, like inflammatory bowel disease. Granulocytes terminate their existence after transmigration through the intestinal wall. Therefore calprotectin is also detectable in feces. Elevated levels of calprotectin have been observed in body fluids such as plasma, saliva, gingival crevicular fluid, stools, and synovial fluid during infection and inflammatory conditions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : 60B8Ag, AI323541, AW546964, B8Ag, BEE22, Caga, Cagb, CFAg, CP-10, GAGB, L1Ag, MRP14, MRP8, p14, p8, S100a8, S100a9
Gene ID : (Mouse) 20201, (Mouse) 20202
Gene symbol : S100a8, S100a9
Protein Aliases : Calgranulin-A, Calgranulin-B, Chemotactic cytokine CP-10, chemotactic S100 protein, Leukocyte L1 complex heavy chain, Leukocyte L1 complex light chain, Migration inhibitory factor-related protein 14, Migration inhibitory factor-related protein 8, MRP-14, MRP-8, Pro-inflammatory S100 cytokine, Protein S100-A8, Protein S100-A9, S100 calcium-binding protein A8, S100 calcium-binding protein A9
UniProt ID (Mouse) P27005, (Mouse) P31725
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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