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Product Specifications
Analytical sensitivity
0.03 µg/mL
Assay range
0.062-0.5 µg/mL
Sample type/volume
Plasma
1 µL
Serum
1 µL
Hands-on time
1 hr 20 min
Time-to-result
1 hr 15 min
Homogenous (no wash)
No
Interassay CV
5.6%
Intraassay CV
4.4%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard/Control
Standard Dilution Buffer
HRP-conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Standard/Control
Standard Dilution Buffer
HRP-conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
SAA
Species (tested)
Mouse
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
HRP
Label or dye
HRP
About This Kit
The Mouse Serum Amyloid A (Ms SAA) ELISA quantitates Ms SAA in mouse serum and plasma. The assay will exclusively recognize both natural and recombinant Ms SAA.
Principle of the method
The Mouse SAA solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
The serum amyloid A family comprises a number of differentially expressed apolipoproteins, acute-phase SAA1 and SAA2, the former being the major component in plasma and constitutive SAAs. Although the liver is the primary site of synthesis of both SAA types extrahepatic production has been reported. The in vivo concentrations increase by as much as 1000 fold during inflammation. Several studies have expressed its importance in the diagnosis and monitoring of various diseases. Pathological SAA values are often detected in association with normal CRP concentrations; SAA rises earlier and more sharply than CRP. Recently, a broader view of SAA expression and function has been emerging.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Gene aliases : Saa-1, Saa1, Saa2
Gene ID : (Mouse) 20208
Gene symbol : Saa1
Protein Aliases : serum amyloid A 2, Serum amyloid A-1 protein
UniProt ID (Mouse) P05366
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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