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Product Specifications

Analytical sensitivity

23 pg/mL

Assay range

23-5,000 pg/mL

Sample type/volume

Serum
50 µL
Plasma
50 µL
Supernatant
50 µL

Hands-on time

1 hr 20 min

Time-to-result

4 hr 45 min

Homogenous (no wash)

No

Interassay CV

<12%

Intraassay CV

<10%

Instrument

Colorimetric Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

2-8°C

Protein name

CD100 (SEMA4D)

Species (tested)

Mouse

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

Mouse Semaphorin 4D/CD100 quantitates mouse Semaphorin 4D/CD100 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant mouse Semaphorin 4D/CD100.

Target information

Semaphorins are neuronal chemorepellants that direct pioneering neurons during nervous system development. CD100 (also known as semaphorin 4d), a 150 kDa homodimer, is a novel semaphorin that induces B cells to aggregate and improves their viability in vitro. CD100 modifies CD40-CD40L B cell signaling by augmenting B cell aggregation and survival and down regulating CD23 expression. CD72 is a lymphocyte receptor for the class IV semaphorin CD100, which is involved in regulating B cell signaling. CD100 is expressed on resting and PHA stimulated T cells. The protein is weakly expressed on NK cells, EBV transformed B cells, monocytes and tumor/peripheral blood T cell lines, and at higher density on activated T cells and B cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : CD100, coll-4, Sema4d, Semacl2, Semaj, Semcl2

Gene ID : (Mouse) 20354

Gene symbol : Sema4d

Protein Aliases : CD100, M-Sema G, Sema J, semaphorin H, Semaphorin-4D, Semaphorin-C-like 2, Semaphorin-J

UniProt ID (Mouse) O09126

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