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Product Specifications
Analytical sensitivity
16 pg/mL
Assay range
16-2,000 pg/mL
Sample type/volume
Serum
100 µL
Supernatant
100 µL
Hands-on time
1 hr 45 min
Time-to-result
24 hr 30 min
Homogenous (no wash)
No
Instrument
Colorimetric Microplate Reader
Product size
10 x 96 Tests
Contents
Capture Antibody: Pre-titrated, purified antibody
Detection Antibody: Pre-titrated, biotin-conjugated antibody
Standard: Recombinant cytokine for generating standard curve and calibrating samples
Coating Buffer: 10X PBS ELISA Coating Buffer
Assay Diluent: 5X Concentrated
Detection Enzyme: Pre-titrated Avidin-HRP
Substrate Solution: Tetramethylbenzidine (TMB) Solution
Certificate of Analysis: Lot-specific instructions for the dilution of antibodies and standards
96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22 and -86)
Detection Antibody: Pre-titrated, biotin-conjugated antibody
Standard: Recombinant cytokine for generating standard curve and calibrating samples
Coating Buffer: 10X PBS ELISA Coating Buffer
Assay Diluent: 5X Concentrated
Detection Enzyme: Pre-titrated Avidin-HRP
Substrate Solution: Tetramethylbenzidine (TMB) Solution
Certificate of Analysis: Lot-specific instructions for the dilution of antibodies and standards
96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22 and -86)
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
TSLP
Species (tested)
Mouse
Assay kit format
Uncoated ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Mouse TSLP Uncoated ELISA Kit contains pre-matched antibody pairs, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse TSLP. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.
Principle of the method
ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Target information
TSLP is a 159 amino acid four helix-bundle hemopoietic cytokine that signals through a heterodimeric receptor complex consisting of the human TSLP receptor and the IL-7R alpha-chain. It impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes, enhances the maturation of CD11c (+) dendritic cells, naive CD4 (+), and CD8 (+) T cells into proallergic effectors, and primes dendritic cells to produce large amounts of IL-12 after CD40 ligand stimulation. TSLP influences the regulation of the positive selection of regulatory T cells and maintenance of peripheral CD4 (+) T cell homeostasis. It induces allergic inflammation by directly activating mast cells and may be involved in the pathophysiology of inflammatory arthritis. It is expressed in heart, liver and prostate.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : Tslp
Gene ID : (Mouse) 53603
Gene symbol : Tslp
Protein Aliases : Thymic stroma-derived lymphopoietin, Thymic stromal lymphopoietin
UniProt ID (Mouse) Q9JIE6
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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