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Mouse Thyroid Stimulating Hormone ELISA Kit
Product Specifications
Analytical sensitivity
0.75 ng/mL
Assay range
1.25-80 ng/mL
Sample type/volume
Plasma
100 µL
Serum
100 µL
Hands-on time
1 hr 20 min
Time-to-result
3 hr 30 min
Homogenous (no wash)
No
Interassay CV
<10%
Intraassay CV
<10%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Shipping conditions
Wet ice
Storage
Refer to product documentation for component specific storage temperature.
Protein name
Thyroid Stimulating Hormone
Protein family
Glycoprotein hormones
Species (tested)
Mouse
Assay kit format
Sandwich ELISA Kit
Label or dye
HRP
About This Kit
The Mouse Thyroid Stimulating Hormone (TSH) ELISA quantitates TSH in serum, plasma, and other biological fluids
The Mouse Thyroid Stimulating Hormone (TSH) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
The four human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. The protein encoded by this gene is the alpha subunit and belongs to the glycoprotein hormones alpha chain family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : aGSU, alpha-GSU, alphaGSU, alphaSU, CG-alpha, Cga, FSHA, GPHA1, GPHalpha, HCG, LHA, Tsha
Gene ID : (Mouse) 12640, (Mouse) 22094
Gene symbol : Cga, Tshb
Protein Aliases : Anterior pituitary glycoprotein hormones common subunit alpha, Follicle-stimulating hormone alpha chain, Follitropin alpha chain, FSH-alpha, Glycoprotein hormones alpha chain, LSH-alpha, Luteinizing hormone alpha chain, Lutropin alpha chain, Thyroid-stimulating hormone alpha chain, thyroid-stimulating hormone subunit beta, Thyrotropin alpha chain, thyrotropin beta chain, thyrotropin subunit beta, TSH-alpha, TSH-B, TSH-beta
UniProt ID (Mouse) P01216
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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