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Product Specifications

Analytical sensitivity

3 ng/mL

Assay range

3-600 ng/mL

Sample type/volume

Plasma
50 µL
Supernatant
50 µL
Serum
50 µL

Hands-on time

1 hr 20 min

Time-to-result

4 hr 45 min

Homogenous (no wash)

No

Interassay CV

<12%

Intraassay CV

<10%

Instrument

Colorimetric Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

2-8°C

Protein name

VISTA

Species (tested)

Mouse

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

Mouse VISTA/B7-H5/PD-1H quantitates mouse VISTA/B7-H5/PD-1H in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant mouse VISTA/B7-H5/PD-1H.

Target information

VISTA (V-domain Ig containing suppressor of T-cell activation), also known as B7-H5 or PD-1H, is a product of the VSIR gene. Although VISTA bears sequence homology to PD-L1 it has a different expression pattern. In both mouse and humans, VISTA is expressed predominantly on hematopoietic cells with the highest densities on myeloid cells, and lower levels on T cells. It is usually absent on B cells. VISTA expressed by antigen presenting cells has been shown to directly suppress proliferation and cytokine production of CD4 and CD8 T cells, making it a target for cancer immunotherapy. The reported ligands of VISTA include CD28H, VSIG-3 and VSIG-8.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : Dies1, PD-1H, VISTA, Vsir

Gene ID : (Mouse) 74048

Gene symbol : 4632428N05Rik

Protein Aliases : differentiation of ESCs 1, Platelet receptor Gi24, V-set domain-containing immunoregulatory receptor, V-set immunoregulatory receptor, V-type immunoglobulin domain-containing suppressor of T-cell activation

UniProt ID (Mouse) Q9D659

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