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Multispecies AKT/ERK Activation InstantOne™ ELISA Kit
FIGURE: 1 / 3
Multispecies AKT/ERK Activation InstantOne™ ELISA Kit
Representative data for AKT/ERK Activation Multispecies InstantOne™ ELISA. Left: MCF7 cells were pre-treated with 10 µM wortmannin washed and unstimulated (-) or stimulated(+) with 10 ng/mL insulin for 10 min. Cells were analyzed for phosphorylated AKT 1/2/3 by InstantOne™ ELISA. Right: A431 cells were pre-treated with 10 µM AG1478 (an EGFR kinase inhibitor) for 3 hours (-) or treated(+) with 10 ng/mL EGF ... View More
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Product Specifications
Sample type/volume
Cell Lysate
20 µL
Hands-on time
1 hr 20 min
Time-to-result
1 hr 10 min
Homogenous (no wash)
No
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Capture Antibody
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
AKT Pan/Erk1/2
Species (tested)
Human
Assay kit format
InstantOne ELISA
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated human, mouse, and hamster ERK 1/2 and AKT 1/2/3 in cell lysates. Detection of rat ERK 1/2 and AKT 1/2/3 is also expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.
Target information
Extracellular signal-regulated kinase (ERK) is the founding member and a key component of the classical Mitogen-Activated Protein Kinase (MAPK) pathway. ERK1 (p44, MAPK3) and ERK2 (p42, MAPK2) are both activated via the MAPK/ERK pathway, which is downstream of various Receptor Tyrosine Kinases (RTKs) or GPCRs. MEK, a Ser/Thr and Tyr kinase, activates ERK by phosphorylation of its TxY motifs, namely Thr202/Tyr204 and Thr185/Tyr187 of ERK1 and ERK2, respectively. Once activated, ERK phosphorylates a variety of proteins that regulate cellular processes such as cell division, proliferation, survival, differentiation, apoptosis, motility, and metabolism. AKT, which exists as multiple isoforms, is one of the principle kinases activated by phosphoinositide 3-kinase (PI3K). Activation of PI3K results in the generation of phosphatidylinositol (3,4,5)-triphosphate (PIP3). These lipid second messengers bind to the pleckstrin homology domain of AKT to promote its translocation to the plasma membrane for activation via phosphorylation at Thr308 and Ser473 by PDK1 and the mTOR TORC2 complex, respectively. Phosphorylation at both these sites is required for full activation of AKT Ser/Thr kinase activity. AKT phosphorylates over 50 known substrates, including GSK3, AS160, PRAS40, TSC 1, TCS 2, Raf-1, Bad, the FOXO family of transcription factors, and PFK2. Due to its established critical role in numerous biological processes, AKT is a frequent target for oncology-related drug development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : AKT, AKT1, AKT2, AKT3, CWS6, ERK, ERK-1, ERK-2, ERK1, ERK2, ERT1, ERT2, HIHGHH, HS44KDAP, HUMKER1A, MAPK1, MAPK2, MAPK3, MPPH, MPPH2, p38, p40, p41, p41mapk, p42-MAPK, P42MAPK, p44-ERK1, p44-MAPK, P44ERK1, P44MAPK, PKB, PKB-ALPHA, PKB-GAMMA, PKBB, PKBBETA, PKBG, PRKBA, PRKBB, PRKBG, PRKM1, PRKM2, PRKM3, RAC, RAC-ALPHA, RAC-BETA, RAC-gamma, RAC-PK-gamma, STK-2
Gene ID : (Human) 207, (Human) 208, (Human) 10000, (Human) 5594, (Human) 5595
Gene symbol : AKT1, AKT2, AKT3, MAPK1, MAPK3
Protein Aliases : AKT1m, ERK-1, ERK-2, ERT1, ERT2, Extracellular signal-regulated kinase 1, Extracellular signal-regulated kinase 2, extracellular signal-related kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase 1, MAP kinase 2, MAP kinase 3, MAP kinase isoform p42, MAP kinase isoform p44, MAPK 1, MAPK 2, Microtubule-associated protein 2 kinase, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 2, Mitogen-activated protein kinase 3, murine thymoma viral (v-akt) homolog-2, p42-MAPK, p44-ERK1, p44-MAPK, PKB, PKB alpha, PKB beta, PKB gamma, Protein kinase Akt-2, Protein kinase Akt-3, Protein kinase B, Protein kinase B alpha, Protein kinase B beta, Protein kinase B gamma, protein tyrosine kinase ERK2, Proto-oncogene c-Akt, putative v-akt murine thymoma viral oncoprotein 2, rac protein kinase alpha, RAC protein kinase beta, RAC-alpha serine/threonine-protein kinase, RAC-beta serine/threonine-protein kinase, RAC-gamma serine/threonine protein kinase, RAC-gamma serine/threonine-protein kinase, RAC-PK-alpha, RAC-PK-beta, RAC-PK-gamma, serine-threonine protein kinase, STK-2, v-akt murine thymoma viral oncogene homolog 1, v-akt murine thymoma viral oncogene homolog 2, v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma), v-akt murine thymoma viral oncogene-like protein 1
UniProt ID (Human) P31749, (Human) Q0VAN1, (Human) Q9Y243, (Human) P28482, (Human) P27361
Performance Guarantee
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