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Multispecies p70 S6 Kinase (Total) InstantOne™ ELISA Kit
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Multispecies p70 S6 Kinase (Total) InstantOne™ ELISA Kit
Representative data for p70 S6 Kinase (Total) Multispecies InstantOne™ ELISA. MCF7 cells were pre-treated with 10 µM wortmannin washed and unstimulated (-) or stimulated with 10 ng/mL insulin for 10 min (+). Cells were analyzed by immunoblotting (left) and InstantOne™ ELISA (right). The intensity of the band at 70 kDa as detected by immunoblot correlates with InstantOne™ ELISA absorption values.
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Product Specifications
Sample type/volume
Cell Lysate
20 µL
Hands-on time
1 hr 20 min
Time-to-result
1 hr 10 min
Homogenous (no wash)
No
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Capture Antibody
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
p70 S6 Kinase
Target sub-specificity
Total
Species (tested)
Human, Mouse
Assay kit format
InstantOne ELISA
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The InstantOne™ ELISA is specifically engineered for accurate measurement of total human and mouse p70 S6K in cell lysates. Detection of rat p70 S6K is expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.
Target information
RPS6KB1 (p70S6K) is a serine/threonine kinase with 2 non-identical kinase catalytic domains which phosphorylates several residues of the S6 ribosomal protein. p70 S6 kinase is predominantly localized in the cytoplasm, and is essential in growth factors regulated cell proliferation, pathways involving cell motility, such as metastases, the immune response, and tissue repair. p70 S6 kinase acts downstream of phosphoinositide (PI) 3-kinase, and its main physiological target is the S6 ribosomal protein, which is involved in upregulation of protein synthesis. The kinase activity of p70 S6 kinase leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding p70 S6 kinase and overexpression are seen in some breast cancer cell lines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Gene aliases : 2610318I15Rik, 4732464A07Rik, 70kDa, AA959758, AI256796, AI314060, p70 S6KA, p70(S6K)-alpha, p70-alpha, p70-S6K, p70/85s6k, p70s6k, PS6K, RPS6KB1, S6K, S6K-beta-1, S6K1, STK14A
Gene ID : (Human) 6198, (Mouse) 72508
Gene symbol : RPS6KB1, Rps6kb1
Protein Aliases : 70 kDa ribosomal protein S6 kinase 1, p70 ribosomal S6 kinase alpha, p70 S6 kinase alpha, p70 S6 kinase, alpha, p70 S6K-alpha, p70 S6KA, p70-S6K 1, P70S6K1, Ribosomal protein S6 kinase beta-1, Ribosomal protein S6 kinase I, ribosomal protein S6 kinase, 70kD, polypeptide 1, 70kDa, S6K, S6K-beta-1, serine/threonine kinase 14 alpha, Serine/threonine-protein kinase 14A
UniProt ID (Human) P23443, (Mouse) Q8BSK8
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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