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Invitrogen
Pregnanediol-3-Glucuronide (PDG) Competitive ELISA Kit
Product Specifications
Analytical sensitivity
0.18 ng/mL
Assay range
0.391-50 ng/mL
Sample type/volume
Fecal Extract
50 µL
Plasma
50 µL
Serum
50 µL
Supernatant
50 µL
Urine
10 µL
Hands-on time
1 hr 20 min
Time-to-result
2 hr 30 min
Homogenous (no wash)
No
Interassay CV
6.4%
Intraassay CV
4.1%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
HRP-Conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
HRP-Conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
-20°C
Protein name
Pregnanediol-3-Glucuronide
Species (tested)
Chemical
Assay kit format
Competitive ELISA
Detector antibody conjugate
HRP
Label or dye
HRP
About This Kit
The Pregnanediol-3-Glucuronide (PDG) ELISA quantitates PDG in extracted serum, plasma, urine, dried fecal extracts or cell culture medium.
Principle of the method
The Pregnanediol-3-Glucuronide (PDG) Competitive ELISA research-use-only kit uses a specifically generated antibody to measure PDG and its metabolites in urine and fecal samples, or in extracted serum and plasma. This kit is not recommended for serum, plasma, or saliva samples without extraction. The kit will quantitatively measure PDG present in diluted buffer samples and tissue culture media samples. A PDG standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. A PDG-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to PDG to each well. After a 2-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound PDG-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
Pregnanediol-3-Glucuronide (PDG) is a natural human metabolite of Pregnanediol generated in the liver by UDP glucuonyltransferase. Glucuronidation is used to assist in the excretion of toxic substances, drugs or other substances that cannot be used as an energy source. Glucuronic acid is attached via a glycosidic bond to the substance, and the resulting glucuronide, which has much higher water solubility than the original substance, is eventually excreted by the kidneys.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases : PDG, C27H44O8
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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