Technical guide & protocol Tech Support

Product Specifications

Analytical sensitivity

0.4 ng/mL

Assay range

0.4-100 ng/mL

Sample type/volume

Plasma
50 µL
Supernatant
50 µL
Serum
50 µL

Hands-on time

1 hr 20 min

Time-to-result

4 hr 45 min

Homogenous (no wash)

No

Interassay CV

<12%

Intraassay CV

<10%

Instrument

Colorimetric Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

2-8°C

Protein name

Angiopoietin 1

Species (tested)

Rat

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

Rat Angiopoietin-1 quantitates rat Angiopoietin-1 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant rat Angiopoietin-1.

Target information

Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : Agpt, Agpt1, Ang-1, Angpt1

Gene ID : (Rat) 89807

Gene symbol : Angpt1

Protein Aliases : ANG-1, Angiopoietin-1

UniProt ID (Rat) O35460

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