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Search Thermo Fisher Scientific
The Rat Interleukin-12 p70 (Rt IL-12 p70) ELISA quantitates IL-12 in rat serum, EDTA plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Rt IL-12 heterodimer.
Principle of the method
The Rat IL-12 p70 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
IL-12 p70, also known as interleukin-12, is a cytokine that plays a critical role in the immune system's response to infections and cancer. It is produced by immune cells, including dendritic cells, macrophages, and B cells, in response to certain pathogens and tumor cells. IL-12 p70 stimulates the production of interferon-gamma and other cytokines, which promote the activation and proliferation of T cells and natural killer cells. IL-12 p70 has been studied as a potential therapeutic target for various diseases and conditions, including infectious diseases, autoimmune disorders, and cancer. It has been shown to enhance immune responses against certain infections and tumors, and to modulate inflammatory responses in autoimmune diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Gene aliases : Il12, Il12a
Gene ID : (Rat) 84405, (Rat) 64546
Gene symbol : Il12a, Il12b
Protein Aliases : CLMF p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, IL-12A, interleukin 12 p35 subunit, interleukin-12 p40, Interleukin-12 subunit alpha, interleukin-12 subunit beta
UniProt ID (Rat) Q9R103
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