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Product Specifications
Analytical sensitivity
<4 pg/mL
Assay range
15.6-1000 pg/mL
Sample type/volume
Plasma
100 µL
Serum
100 µL
Supernatant
100 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr
Homogenous (no wash)
No
Interassay CV
4.9%
Intraassay CV
3.9%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Standard Dilution Buffer
Biotinylated Detection Antibody
Streptavidin-HRP
HRP Diluent
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Standard Dilution Buffer
Biotinylated Detection Antibody
Streptavidin-HRP
HRP Diluent
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
IL-18
Protein family
Cytokines & Receptors
Species (tested)
Rat
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Rat Interleukin 18 (Rt IL-18) ELISA quantitates Rt IL-18 in rat serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Rt IL-18.
Principle of the method
The Rat IL-18 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
The protein encoded by this gene is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein can specifically interact with this cytokine, and thus negatively regulate its biological activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Gene aliases : Igif, IL-18, Il18
Gene ID : (Rat) 29197
Gene symbol : Il18
Protein Aliases : IFN-gamma-inducing factor, IL-1 gamma, IL-18, Interferon gamma-inducing factor, interferon-gamma-inducing factor, Interleukin-1 gamma, Interleukin-18
UniProt ID (Rat) P97636
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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