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Product Specifications
Analytical sensitivity
0.94 mIU/mL
Assay range
1.56-100 mIU/mL
Sample type/volume
Plasma
100 µL
Serum
100 µL
Hands-on time
1 hr 20 min
Time-to-result
3 hr 30 min
Homogenous (no wash)
No
Interassay CV
<10%
Intraassay CV
<10%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Shipping conditions
Wet ice
Storage
Refer to product documentation for component specific storage temperature.
Protein name
Luteinizing Hormone
Protein family
Glycoprotein hormone
Species (tested)
Rat
Assay kit format
Sandwich ELISA Kit
Label or dye
HRP
About This Kit
The Rat Luteinizing Hormone (LH) ELISA quantitates LH in serum, plasma, and other biological fluids
The Rat Luteinizing Hormone (LH) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
LH is a member of the glycoprotein hormone family, Glycoprotein hormones are heterodimers consisting of a common alpha subunit and an unique beta subunit which confers biological specificity. LH is expressed in the pituitary gland and promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids. The genes for the beta chains of chorionic gonadotropin and for luteinizing hormone are contiguous on chromosome 19q13.3. Mutations in this gene are associated with hypogonadism which is characterized by infertility and pseudohermaphroditism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : Cga, Cga1, Lhb
Gene ID : (Rat) 116700, (Rat) 25329
Gene symbol : Cga, Lhb
Protein Aliases : Anterior pituitary glycoprotein hormones common subunit alpha, Follicle-stimulating hormone alpha chain, Follitropin alpha chain, FSH-alpha, Glycoprotein hormones alpha chain, glycoprotein hormones, alpha subunit, LH-B, LSH-alpha, LSH-B, LSH-beta, Luteinizing hormone (lutropin) subunit beta, Luteinizing hormone alpha chain, Luteinizing hormone subunit beta, Lutropin alpha chain, Lutropin beta chain, Lutropin subunit beta, pituitary hormone alpha subunit, Thyroid-stimulating hormone alpha chain, Thyrotropin alpha chain, TSH-alpha
UniProt ID (Rat) P11962, (Rat) P01230
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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