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Product Specifications

Sample type/volume

Cell Lysate
20 µL

Hands-on time

1 hr 20 min

Time-to-result

1 hr 10 min

Homogenous (no wash)

No

Instrument

Colorimetric Microplate Reader

Product size

96 Tests

Contents

Capture Antibody
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal

Shipping conditions

Wet ice

Storage

2-8°C

Protein name

p38 MAPK alpha

Target site-specificity

Phosphorylated Thr180, Phosphorylated Tyr182

Species (tested)

Human, Mouse

Assay kit format

InstantOne ELISA

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated human and mouse p38 MAPK in cell lysates. Detection of rat p38 MAPK is expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.

Target information

MAPK14 (p38 alpha) is a member of the stress-activated protein kinase class of MAPKs. The p38 MAPK cascade regulates a variety of cellular responses to stress, inflammation, and other signals. p38 MAPK is relatively inactive in the nonphosphorylated form and becomes rapidly activated by dual phosphorylation of a Thr-Gly-Tyr motifs. There are four isoforms of p38 MAPK, which differ in their tissue expression and affinity for upstream activators and downstream effectors. When cells are exposed to tumor necrosis factor, interleukin-1, heat shock, or other activating stimuli, activation of MAPK kinase-3 occurs by phosphorylation. Activated MAPK kinase-3/6 phosphorylate each residue of Thr180 and Tyr182 in p38 MAPK. Phospho-p38 MAPK activates ATF-2, CHOP-1, MEF-2 and other transcription factors through phosphorylation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Gene aliases : Crk1, CSBP, CSBP1, CSBP2, CSPB1, EXIP, MAPK14, MXI2, p38, p38-alpha, p38a, p38ALPHA, p38MAPK, PRKM14, PRKM15, RK, SAPK2A

Gene ID : (Human) 1432, (Mouse) 26416

Gene symbol : MAPK14, Mapk14

Protein Aliases : CRK1, CSAID-binding protein, Csaids binding protein, cytokine suppressive anti-inflammatory drug binding protein, cytokine suppressive anti-inflammatory drug binding protein 1, Cytokine suppressive anti-inflammatory drug-binding protein, MAP kinase 14, MAP kinase MXI2, MAP kinase p38 alpha, MAPK 14, MAX-interacting protein 2, mitogen activated protein kinase 14, Mitogen-activated protein kinase 14, Mitogen-activated protein kinase p38 alpha, p38 alpha, p38 MAP kinase, p38 MAP kinase alpha, p38 MAPK, p38 mitogen activated protein kinase, p38alpha Exip, SAPK2a, Stress-activated protein kinase 2a, tRNA synthetase cofactor p38

UniProt ID (Human) Q16539, (Mouse) P47811

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