Qubit Flex Fluorometer

The Qubit Flex Fluorometer shares common features with the Qubit 4 model: It is accurate, specific, sensitive, simple, and fast; it requires as little as 1 µL of sample, even if highly diluted; it offers an onboard Reagent Calculator to help in preparing your working solution; it works with a broad range of Qubit Assays  and applications; and it can measure raw fluorescence in Fluorometer mode.

In addition, the Qubit Flex offers these additional features:

By quantifying up to eight samples at a time, the Qubit Flex Fluorometer can increase your throughput. If you have more than eight samples, simply load them into additional tube strips and process them sequentially.

The graph shows time to data, including sample prep and measurement, for a given number of samples with the Qubit 4 or Flex Fluorometer as well as a competitor’s instrument. The Qubit Flex Fluorometer can reduce time to data by 50% or more for larger numbers of samples.

The Qubit Flex Fluorometer reduces time to data by up to 50%. A time study comparing the Qubit Flex Fluorometer to the Qubit 4 Fluorometer and another supplier’s fluorometer, using the Qubit 1X dsDNA HS Assay Kit, showed time-to-data reduced by up to 50% with up to 96 samples. 

Tip: For high-throughput quantification, consider using a microplate reader with fluorescence capabilities. The Thermo Scientific Varioskan ALF Multimode Microplate Reader and Varioskan LUX Multimode Microplate Reader are compatible with Quant-iT assays to quantify the same analytes as Qubit Fluorometers and assays. For a comparison, see our RNA/DNA Quantification page.

All Qubit Fluorometers feature an onboard Reagent Calculator, which calculates the volume of reagent and buffer to use when preparing your working solution, depending on the number of samples and standards you are testing. It can be used to determine master mix volumes for samples and standards.

The Qubit Flex Fluorometer offers three additional calculators to streamline your workflow. The Assay Range Calculator displays the core sample concentration range for which it is most accurate, based on the sample volume used to quantitate. It will also display the extended low and high concentration ranges based on the quantitation sample volume.

To aid your transition to downstream applications, two calculators help you manage assay sample data and perform common unit conversions and dilutions. The Molarity Calculator allows you to determine the molarity of a sample based on nucleic acid length and the measured concentration. For sequencing applications, the Normalization Calculator replaces the spreadsheet often used to normalize samples during library preparation. For each run, it recommends how much sample and buffer to add to reach a desired, normalized mass, concentration, or molarity.

Qubit Flex Fluorometers come equipped with an optional add-on Security, Audit, and e-Signature (SAE) software that supports compliance to 21 CFR Part 11.