TaqMan® Primers and Probes Support—Troubleshooting

You can check the paperwork that came with your order for the dye label of your TaqMan probe. Alternatively you can contact Customer Service at genomicorders@thermofisher.com with your order number if you cannot find the COA that came with your probe.

There are several reasons why a custom TaqMan probe may not be working. If the probe sequence is new and has not been tested before, please check for the following:

• Run the probe with a positive control to check for amplification.
  
• Were different concentrations of probe tested?
• Is there any product visible on an agarose gel?
  
• How was the probe designed? Was it checked for specificity for the relevant species?
  
• Check that the probe reporter dye has been set correctly in the software.

If the probe sequence has been used successfully in the past, please check for the following:

• Run the probe with a positive control to check for amplification.
• Run a side-by-side test on the same plate with probe from a previous lot to rule out problems from sample or master mix.
• Check that the probe reporter dye has been set correctly in the software.

If you suspect a problem with your custom TaqMan probe, please send your data and order information to techsupport@thermofisher.com so that we can review the issue further.